Fig. 7: UV-VIS spectrum of Olanzapine in Basic Degradation Oxidative Degradation of Olanzapine Accurately weighed 10 mg bulk drug was taken in 10 ml volumetric flask. Then the volume was made with 3% H 2O2 and refluxed for 5 h at 60ºC. Samples were withdrawn according to protocol. From the drawn samples 25μg/ml solution were prepared and subjected for analysis. The representative UV-VIS. Four analytical methods have been developed for the quality control of pharmaceutical formulations containing the novel antipsychotic drug, olanzapine: high performance liquid chromatography (HPLC), capillary zone electrophoresis (CZE), derivative spectrometry and linear voltammetry. The ultraviolet-visible reference spectra presented here were obtained by the use of double- beam spectrophotometers with sample solutions prepared as specified in the individual mono- graphs. The overlaid absorption spectrum of olanzapine and its condensation product with DMAB in methanol are shown with that of DMAB alone in Fig. 1. All three compounds had significant absorption in the UV region. Most absorption of olanzapine occurred around 250 Olanzapine, sold under the trade name Zyprexa among others, is an atypical antipsychotic primarily used to treat schizophrenia and bipolar disorder. For schizophrenia, it can be used for both new onset disease and long term maintenance. It is taken by mouth or by injection into a muscle. Determination of olanzapine by spectrophotometry using permanganate 541 Sulphuric acid (0.1 and 2 M) Concentrated acid (S.D. Fine Chem, Mumbai, India, Keywords: Olanzapine, Derivative spectrophotometric, First derivative spectrum,Second derivative spectrum. INTRODUCTION Olanzapine, a thienobenzodiazepine derivative with olanzapine bulk drug, where the initial mobile phase consisted of mobile phase A (10 mM disodium hydrogen phosphate, pH adjusted to 7.4 with orthophosphoric acid) and mobile phase B (acetonitrile) in a ratio of 70:30 (v/v) for 15 min. As can be seen from Fig. 3, the UV spectrum of olanzapine has λ max of 226 nm and 271 nm, and the spectrum of protonated olanzapine had a maximum at 261 nm. We were able to select chromatographic con ditions under which the retention time of olanzapine synthesized by a reported procedure [8] differed from the retention time of protonated olanzapine. In the former case, the retention time was. Figure 1 Spectrum of olanzapine treated with DDQ solution It is evident from Figure 1. That the olanzapine drug treated with DDQ solution has maximum absorbance at.